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rabbit anti–type x collagen polyclonal antibody  (Millipore)


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    Millipore rabbit anti–type x collagen polyclonal antibody
    Rabbit Anti–Type X Collagen Polyclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti%E2%80%93type+x+collagen+polyclonal+antibody/rabbit+anti+collagen+iv/pmc04819196-143-36-43
    Average 90 stars, based on 1 article reviews
    rabbit anti–type x collagen polyclonal antibody - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Activity Assay:

    Article Title: A novel fibroblast growth factor receptor 1 inhibitor protects against cartilage degradation in a murine model of osteoarthritis
    Article Snippet: .. After being deparaffinized using xylene and deprived of endogenous peroxidase activity with 3% H 2 O 2 , and antigen retrieval with 0.1% trypsin, sections were incubated with rabbit anti–MMP-13 polyclonal antibody (1:200 dilution; Abcam), rabbit anti–type X collagen polyclonal antibody (1:200 dilution; Millipore), rabbit anti- cleaved caspase-3 polyclonal antibody (1:100; Boster) overnight at 4 °C. ..

    Incubation:

    Article Title: A novel fibroblast growth factor receptor 1 inhibitor protects against cartilage degradation in a murine model of osteoarthritis
    Article Snippet: .. After being deparaffinized using xylene and deprived of endogenous peroxidase activity with 3% H 2 O 2 , and antigen retrieval with 0.1% trypsin, sections were incubated with rabbit anti–MMP-13 polyclonal antibody (1:200 dilution; Abcam), rabbit anti–type X collagen polyclonal antibody (1:200 dilution; Millipore), rabbit anti- cleaved caspase-3 polyclonal antibody (1:100; Boster) overnight at 4 °C. ..

    Article Title: Genetic Inhibition of Fibroblast Growth Factor Receptor 1 in Knee Cartilage Attenuates the Degeneration of Articular Cartilage in Adult Mice
    Article Snippet: .. Sections were incubated overnight with rabbit anti–FGFR-1 polyclonal antibody (1:100 dilution; Abcam), rabbit anti–FGFR-3 polyclonal antibody (1:100 dilution; Santa Cruz Biotechnology), rabbit anti–MMP-13 polyclonal antibody (1:50 dilution; ProteinTech), and rabbit anti–type X collagen polyclonal antibody (1:200 dilution; Calbiochem-Merck). ..



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    The incorporation of CII was associated with a reduction of <t>COLX</t> synthesis, and in combination with HyA, was found to increase the accumulation of COLII in the matrices by day 28. The expression of COLII ( A ), COLX ( B ) and GAPDH (housekeeper) ( C ) proteins was determined for scaffolds seeded with human MSCs from donors 1, donor 2 and donor 3 after 28 days in culture. COLII and COLX protein expression was also determined on cell-free scaffolds ( D ). The relative protein-band intensity of COLII and COLX was quantified and normalised to GAPDH ( E ).
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    Image Search Results


    The incorporation of CII was associated with a reduction of COLX synthesis, and in combination with HyA, was found to increase the accumulation of COLII in the matrices by day 28. The expression of COLII ( A ), COLX ( B ) and GAPDH (housekeeper) ( C ) proteins was determined for scaffolds seeded with human MSCs from donors 1, donor 2 and donor 3 after 28 days in culture. COLII and COLX protein expression was also determined on cell-free scaffolds ( D ). The relative protein-band intensity of COLII and COLX was quantified and normalised to GAPDH ( E ).

    Journal: Bioengineering

    Article Title: Highly Porous Type II Collagen-Containing Scaffolds for Enhanced Cartilage Repair with Reduced Hypertrophic Cartilage Formation

    doi: 10.3390/bioengineering9060232

    Figure Lengend Snippet: The incorporation of CII was associated with a reduction of COLX synthesis, and in combination with HyA, was found to increase the accumulation of COLII in the matrices by day 28. The expression of COLII ( A ), COLX ( B ) and GAPDH (housekeeper) ( C ) proteins was determined for scaffolds seeded with human MSCs from donors 1, donor 2 and donor 3 after 28 days in culture. COLII and COLX protein expression was also determined on cell-free scaffolds ( D ). The relative protein-band intensity of COLII and COLX was quantified and normalised to GAPDH ( E ).

    Article Snippet: The membrane was incubated at 4 °C overnight with a 1:1000 solution of diluted rabbit monoclonal [EPR12268] anti-type II collagen (COLII) antibody (Abcam, ab188570), 1:1000 diluted rabbit polyclonal anti-type X collagen (COLX) antibody (Abcam, ab58632) and 1:5000 rabbit polyclonal anti-GAPDH (GAPDH) antibody (Abcam, ab9485).

    Techniques: Expressing